Silvester, Warwick B.; Sollins, Phillip; Verhoeven, Thomas; Cline, Steven P. 1982. Nitrogen fixation and acetylene reduction in decaying conifer boles: effects of incubation time, aeration, and moisture content. Canadian Journal of Forest Research. 12(3): 646-652.
Free-living microaerophiles fixed '51\12 and reduced acetylene in fallen tree boles at two old-growth Pseudotsuga menziesiistands in western Oregon. Acetylene reduction was most rapid under an atmosphere of 2-10% 02, whereas under prolongedanaerobic conditions it was at or below detection limits. Acetylene reduction rates increased up to fourfold during long-termincubations in acetylene (> 12 h). Ratios of acetylene reduction to N2 fixation frequently exceeded 6.0 during such long-termincubations but averaged 3.5 when samples were incubated