Publication Title: Identification of fungi decomposing woody conifer roots in Oregon using molecular methods [Abstract]
Year: 2001 Status: Published Publication Type: Abstract
H. J. Andrews Publication Number: 3138
Citation: Vandegrift, V. Eleanor; Chen, Hua; Horton, Thomas R.; Harmon, Mark E. 2001. Identification of fungi decomposing woody conifer roots in Oregon using molecular methods [Abstract]. In: The Ecological Society of America 86th annual meeting: Keeping all the parts: preserving, restoring and sustaining complex ecosystems; Madison, WI. Washington, DC: The Ecological Society of America: 225.
Online PDF: http://andrewsforest.oregonstate.edu/pubs/pdf/pub3138.pdf
Abstract: Previous studies have indicated that roots from five tree species (Picea sitclwnsis, Tsuga heterophylla, Pseudotsuga menziesii, Pinus ponderosa, and Pious contorta) found across an environmental gradient in Oregon, decompose at different rates. These differences are not explained by moisture, temperature, or wood chemistry. We used molecular techniques to examine wood rotting fungi found in roots of these tree species and test if differences in fungal community structure might explain observed differences in decomposition. Root samples were retrieved from trees cut 5-15 years prior to sampling. Two tree species were sampled at each of three locations across Oregon. Hyphae samples were collected directly from roots, and fungi were cultured from sterile root chips. Eight hundred fifty samples have been obtained, representing 350 hyphae and 500 cultured samples. To analyze genetic diversity of the samples, Internal Transcribed Spacer - Restriction Fragment Length Polymorphism (ITS-RFLP) patterns are compared. A database of ITS-RFLP patterns obtained from sporocarps is used to match and identify the fungi from the root samples. DNA sequences and taxon specific phylogenetic sequence databases are employed to illustrate potential taxonomic relationships between remaining undescribed taxa. Multivariate analyses are used to compare differences in fungal composition between tree species. ITS-RFLP patterns indicate that there are different compositions of fungal communities compared across the five species with P. ponderosa and P. contorta having fungal communities that are most similar.
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